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KMID : 0043319900130040325
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Archives of Pharmacal Research
1990 Volume.13 No. 4 p.325 ~ p.329
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Comparison of Several Procedures for the Preparation of Synaptosomal Plasma Membrane Vesicles
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Yun I
Kim YS/Yu SH/Chung IK/Kim IS/Baik SW/Cho GJ/Chung YZ/Kim SH/Kang JS
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Abstract
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Synaptosomal plasma membrane vesicles (SPMV) were isolated from fresh bovine cerebral cortex by several well-known procedures, and the best procedure was selected by enzymatic and morphological standards. The SPMV isolated by the modified procedure of Smith and Loh showed the highest purity. The specific activities of Na, K-ATPase, acetylcholinesterase and 5’’-nucleotidase were about 5.6-fold, 2.5-fold and 3.3-fold, respectively, enriched in the plasma membrane fraction as compared to those of the crude homogenate. Electron microscopic examination also showed that the membranes were in vesicular form.
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